學系成員

Publication:

1. Chi, Y.-H. and Huang, L.-F. (2022) Current strategies to improve yield of recombinant protein production in rice suspension cells. Processes. 10(6):1120. https://doi.org/10.3390/pr10061120  (SCI, Impact Factor: 3.352).

2. Nguyen, T.M., Lu, C.A., Huang, L.F.* (2022) Applications of CRISPR/Cas9 in a rice protein expression system via an intron-targeted insertion approach. Plant Science, 315:111132. https://doi.org/10.1016/j.plantsci.2021.111132 (SCI, Impact Factor: 4.729).

3. Wu, C.L., Lin, L.F., Hsu, H.C., Huang, L.F., Hsiao, C.D., Chou, M.L. (2021) Saussurea involucrata (Snow Lotus) ICE1 and ICE2 orthologues involved in regulating cold stress tolerance in transgenic Arabidopsis. Int. J. Mol. Sci., 22, 10850. https://doi.org/10.3390/ijms221910850 (SCI, Impact Factor: 5.924).

4. Sinaga, D.S., Ho, S.L, Lu. C.A., Yu, S.M., Huang, L.F.* (2021) Knockdown expression of a MYB-related transcription factor gene, OsMYBS2, enhances production of recombinant proteins in rice suspension cells. Plant Methods, 25(17):99. doi: 10.1186/s13007-021-00799-2 (SCI, Impact Factor: 4.993). 

5. Huang, L.F.*, Sinaga, D.S., Tan, C.C., Hsieh, S.J.M., Huang, C.H. (2021) Expression of recombinant human octamer-binding transcription factor 4 in rice suspension cells. Int. J. Mol. Sci., 22: 1409. https://doi.org/10.3390/ijms22031409 (SCI, Impact Factor: 5.924).

6. Chuang, J.N., Diao, P.Y., Huang, W.S., Huang, L.F., Senapati, S., Chang, H.C. and Sun, Y.M.* (2020) Novel homogeneous anion exchange membranes for reproducible and sensitive nucleic acid detection via current–voltage characteristic measurement, ACS Applied Materials and Interfaces, 49: 54459–54472 (SCI, Impact Factor: 9.229).

7. Huang, L.F.*, Liu, Y.K., Su, S.-C., Lai, C.-C., Wu, C.-R., Chao, T.-J. Yang, Y.H. (2020) Genetic engineering of transitory starch accumulation by knockdown of OsSEX4 in rice plants for enhanced bioethanol production, Biotechnol Bioeng, 117: 933-944 (SCI, Impact Factor: 4.53).

8. Liu, Y.K., Lu, C.W., Chang, J.Y., Lu, C.F., Tan, C.C. and Huang, L.F.* (2018) Optimization of the culture medium for recombinant protein production under the control of the αAmy3 promoter in a rice suspension-cultured cell expression system, Plant Cell, Tissue and Organ Culture, 132: 383–391. (SCI, Impact Factor: 2.711).

9. Huang, L.F., Lin, K.H., He, S.L., Chen, J.L., Jiang, J.Z., Chen, B.H., Hou, Y.S., Chen, R.S., Hong, C.Y. and Ho, S.H.* (2016) Multiple patterns of regulation and overexpression of a ribonuclease-like pathogenesis-related protein gene, OsPR10a, conferring disease resistance in rice and Arabidopsis. PLoS ONE, 11(6): e0156414. doi:10.1371/journal.pone.0156414. (SCI, Impact Factor: 3.240).

10. Huang, C.K., Shen, Y.L., Huang, L.F., Wu, S.J., Yeh, C.H., Lu, C.A.* (2016) The DEAD-Box RNA Helicase AtRH7/PRH75 Participates in Pre-rRNA Processing, Plant Development and Cold Tolerance in Arabidopsis. Plant Cell Physiol., 57(1):174-91. doi: 10.1093/pcp/pcv188. (SCI, Impact Factor: 4.927).

11. Huang, L.F.*, Tan, C.C., Yeh, J.F., Liu, H.Y., Liu, Y.K., Ho, S.L., Lu, C.A. (2015) Efficient secretion of recombinant proteins from rice suspension cultured cells modulated by the choice of signal Peptide. PLoS ONE, 10(10): e0140812. doi:10.1371/journal.pone.0140812 (SCI, Impact Factor: 3.240).

12. Huang, L.F.*; Lin, J.Y.; Pan, K.Y.; Huang, C.K.; Chu, Y.K. (2015) Overexpressing Ferredoxins in Chlamydomonas reinhardtii increase starch and oil yields and enhance electric power production in a photo microbial fuel cell. Int. J. Mol. Sci., 16: 19308-19325, Link: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581298/ (SCI, Impact Factor: 5.924).

13. Huang, C.K., Lo, P.C., Huang, L.F., Wu, S.J., Yeh, C.H., Lu, C.A.* (2015) A single-repeat MYB transcription repressor, MYBH, participates in regulation of leaf senescence in Arabidopsis. Plant Mol Biol, 88(3):269-86. doi: 10.1007/s11103-015-0321-2. (SCI, Impact Factor: 4.076).

14. Liu, Y.K., Li, Y.T., Lu, C.F., Huang, L.F.* (2015) Enhancement of recombinant human serum albumin in transgenic rice cell culture system by cultivation strategy. New Biotechnology, 32(3): 328-334 (SCI, Impact Factor: 5.079).

15. Lin. Y.H., Huang. L.F., Hase, T., Huang, H. E., Feng, T.Y. (2015) Expression of plant ferredoxin-like protein (PFLP) enhances tolerance to heat stress in Arabidopsis thaliana. New Biotechnolgy, 32(2): 235-242 (SCI, Impact Factor: 5.079).

16. Chou, W.L., Huang, L.F., Fang, J.C., Yeh, C.H., Hong, C.Y., Wu, S.J., Lu, C.A. (2014) Divergence of the expression and subcellular localization of CCR4‑associated factor 1 (CAF1) deadenylase proteins in Oryza sativa. Plant Mol Biol, 85(4-5): 443-458 (SCI, Impact Factor: 4.076).

17. Chen, W.C., Lai, Y.A., Lin, Y.C., Ma, J.W., Huang, L.F., Yang, N.S., Ho, C.T., Kuo, S.C., Way, T.D. (2013) Curcumin suppresses Doxorubicin-induced epithelial-mesenchymal transition via the inhibition of TGF-β and PI3K/AKT signaling pathways in triple-negative breast cancer cells. Journal of Agricultural and Food Chemistry, 61(48): 11817~11824 (SCI, Impact factor 5.279).

18. Chen. C.C., Chen, Y.; Hsi, Y.T.; Chang, C.S.; Huang, L.F., Ho, C.T., Way T.D., Kao, J.Y. (2013) Chemical constituents and anticancer activity of Curcuma zedoaria Roscoe essential oil against non-small cell lung carcinoma cells in vitro and in vivo. Journal of Agricultural and Food Chemistry, 61(47): 11418~11427 (SCI, Impact factor 5.279).

19. Lin, Y.H., Pan, K.Y., Hung, C.H., Huang, H.E., Chen, C.L., Feng, T.Y., Huang, L.F.* (2013) Over-expression of Ferredoxin, PETF, enhances tolerance to heat stress in Chlamydomonas reinhardtii. Int. J. Mol. Sci.,14(10) 20913-20929. Link: http://www.mdpi.com/1422-0067/14/10/20913 (SCI, Impact factor: 5.924).

20. Kuo, Y.C., Tan, C.C., Ku, J.T., Hsu, W.C., Su, S.C., Lu, C.A., Huang, L.F.* (2013) Improving Pharmaceutical Protein Production in Oryza sativa. Int. J. Mol. Sci., 14: 8719-8739. Link: http://www.mdpi.com/1422-0067/14/5/8719 (SCI, Impact factor: 5.924).

21. Ho, S.L., Huang, L.F., Lu, C.A., Wang, C.C., Chen, J and Yu, S.M. (2013) Sugar starvation- and GA-inducible calcium-dependent protein kinase 1 feedback regulates GA biosynthesis and activates a 14-3-3 protein to confer drought tolerance in rice seedlings. Plant Mol Biol, 81:347-361 (equal contribution as the first author, SCI, Impact Factor: 4.076).

22. Liu, Y.K., Huang, L.F., Ho, S.L., Liao, C.Y., Liu, H.Y., Lai, Y.H., Yu, S.M. and Lu, C.A. (2012) Production of mouse granulocyte-macrophage colony stimulating factor by Gateway technology and transgenic rice cell culture. Biotechnology & Bioengineering, 109:1239-1247 (SCI, Impact Factor: 4.53).

23. Lo, Y.C., Huang, L.F., Cheng, C.L., Chen, J. and Chang, J.S. (2011) Using a starch-rich mutant of Arabidopsis thaliana as feedstock for fermentative hydrogen production. Bioresource Technology, 102:8543-8546. (SCI, Impact factor: 9.642).

24. Huang, C.K., Huang, L.F., Huang, J.J., Wu, S.J., Yeh, C.H., Lu, C.A. (2010) A DEAD-box protein, AtRH36, is essential for female gametophyte development and is involved in rRNA biogenesis in Arabidopsis. Plant and Cell Physiology, 51:694-706 (SCI, Impact factor: 4.927).

25. Lin, H.H., Huang, L.F., Su, H.C., Jeng. S.T. (2009) Effects of the multiple polyadenylation signal AAUAAA on mRNA 3'-end formation and gene expression. Planta, 230:699-712 (equal contribution as the first author, SCI, Impact Factor: 4.116).

26. Lu, C.A., Lin, C.C., Lee, K.W., Chen, J.L., Huang, L.F., Ho, S.L., Liu, H.J., Hsing, Y.I. and Yu, S.M. (2007) The SnRK1A protein kinase plays a key role in sugar signaling during germination and seedling growth of Rice. Plant Cell, 19: 2484-2499 (SCI, Impact Factor: 11.277).

27. Huang, L.F., Bocock, P.N., Davis , J.M. and Koch, K.E. (2007) Invertase regulation: a “suite” of transcriptional and post-transcriptional mechanisms. Functional Plant Biology, 34:499-507 (SCI, Impact Factor: 3.101).

28. Kohorn, B.D., Kobayashi, M., Johansen, S., Riese, J., Huang, L.F., Koch, K., Fu, S., Dotson, A. and Byers, N. (2006) An Arabidopsis cell wall-associated kinase required for invertase activity and cell growth. Plant Journal, 46:307-316 (SCI, Impact Factor: 6.486).

29. Huang, L.F., Liu, Y.K., Lu, C.A., Hsieh, S.L. and Yu, S.M. (2005) Production of human serum albumin by sugar starvation induced promoter and rice cell culture. Transgenic Research, 14:569-581 (SCI, Impact Factor: 2.788).

30. Chiang, C.M., Yeh, F.S., Huang, L.F., Tseng, T.H., Chung M.C., Wang C.S., Lur, H.S., Shaw, J.F. and Yu, S.M. (2005) Expression of a bi-functional and thermostable amylopullulanase in transgenic rice seeds leads to autohydrolysis and altered composition of starch. Molecular Breeding, 15:125-143 (SCI, Impact Factor: 2.589).

31. Chen, M.H., Huang, L.F., Li, H.M., Chen, Y.R, Yu, S.M. (2004) Signal peptide-dependent targeting of a rice α-amylase and cargo proteins to plastids and extracellular compartments of plant cells. Plant Physiology, 135: 1367-1377 (equal contribution as the first author, SCI, Impact Factor: 8.34).

* indicates corresponding author

 註: Impact Factor引用來源：Journal Citation Reports™ 2020

歷年科技部獎助之計畫:

執行期間    個人型計畫題目

2022-2025    CRISPR基因編輯技術於水稻分子農場之應用

2021-2022    應用CRISPR-Cas9系統編輯水稻基因以優化重組蛋白表現量

2017-2021    水稻細胞表現人類幹細胞轉錄因子Oct4重組蛋白的研發與應用

2014-2017    OsSEX4在水稻葉片澱粉降解角色的研究與應用

2013-2014    植物特有蛋白質醣基化基因在水稻的探討與應用

2010-2011    以甘藷作為植物分子農場大量生產顆粒細胞-巨噬細胞集落刺激因 子(GM-CSF)

2009-2010    改造水稻細胞醣化作用系統以生產高價值重組蛋白

2008-2009    14-3-3蛋白與植物葉片澱粉代謝相關酵素結合之功能分析

Patent

1. US 10,041,080 B2 MODIFIED PROMOTER SEQUENCE AND APPLICATION THEREO (2018/8/7)

2. 臺灣發明專利 , 發明第I530503號, [修飾的啟動子序列及其應用]，專利權人：元智大學；發明人：黃麗芬、陳鵬文、余淑美 (2016/04/21)

3. 臺灣發明專利 , 發明第I792184號, [ 均質相陰離子交換膜、生物傳感膜 ]，專利權人：元智大學；發明人： 孫一明、黃麗芬、莊介寧、黃文杉 (2023/02/11)

3. 臺灣發明專利 , 發明第I821020號, [ 生物傳感膜 ]，專利權人：元智大學；發明人： 孫一明、黃麗芬、莊介寧、黃文杉 (2023/11/01)

-2023/6/19 指導助理梁凱茵參加【2023第十四屆桃園四校生命科技暨永續創意學術研討會】海報競賽(2023/6/19)，獲得生化與生醫工程組特優(第一名)。

-2021/11/18於亞東醫院14樓國際會議廳參與「110 年度亞東元智智慧生技醫療創客平台成果發表會」，與曾一修醫師共同演講發表【天山雪蓮懸浮細胞萃取液對HK-2細胞上草酸鈣結晶引起的TNF-α與黏附蛋白的影響】，並獲最佳海報獎。

-2019/5/31指導助理辛娜嘉參加中央大學主辦之【2019年第十二屆桃園四校生命科技與工程研討會】壁報比賽，獲得[生技工程組]特優(第一名)。

-2018年12月帶領跨校團隊，包含元智、中央、慈濟和長庚學生助理共8人，獲教育部「2018生醫產業與新農業創新創業競賽」，醫藥B組金牌第一。

-2018年6月指導外籍學生古瑪莉，參加2018第11屆桃園四校生命科學領域聯合學術研討會海報競賽，獲生化生醫組第三名甲等。

-2017年9月於韓國POSTECH生技中心參與第一屆植物製藥物亞洲會議，獲得最佳海報獎 (Best Poster Award)。 

-2016年指導張姓研究生參加中央大學主辦之【2016第九屆桃園四校生命科技領域聯合學術研討會】壁報比賽，獲得[生技工程組]特優(第一名)。

-104學年度榮獲元智大學[青年學者研究獎] 

-2015年指導褚姓研究生參加中原大學主辦之【2015第八屆桃園四校生命科技領域聯合學術研討會】壁報比賽，獲得[生化生醫工程組]優選(第二名)。

-2014年指導褚姓研究生參加 10th International Symposium on Biocatalysis and Agricultural Biotechnology (ISBAB)，榮獲"Best poster Award" 。 

-2014年指導劉姓研究生榮獲"元智大學生技所第六屆學生學術研討會論文競賽"二年級第一名。 

-2014年指導劉姓研究生榮獲"2014第19屆生物技術暨生物工程研討會"壁報論文競賽-優勝。 

-2014年指導劉姓研究生參加長庚大學主辦之【2014第七屆桃園四校生命科技領域聯合學術研討會】壁報比賽，獲得[生化生醫工程組]優選。

-2013年指導林姓研究生榮獲"2013第18屆生物技術暨生物工程研討會"壁報論文競賽-優勝。 

-2013年指導外籍研究生丹寧榮獲"2013第18屆生物技術暨生物工程研討會"英語口頭論文競賽-佳作。 

-2013年指導郭姓研究生榮獲"2013第18屆生物技術暨生物工程研討會"英語口頭論文競賽-第一名。 

-2013年指導蘇姓研究生榮獲"第六屆桃園四校生命科技領域聯合學術研討會"論文海報競賽-生技工程組第一名。 

-2013年指導外籍研究生丹寧榮獲"第六屆桃園四校生命科技領域聯合學術研討會"論文海報競賽-生技工程組第三名。 

-2012年指導譚姓學生榮獲"2012中華植物學會年會"壁報論文競賽優等暨最佳人氣海報。  

-2012年指導吳姓研究生榮獲"第十七屆生化工程研討會"壁報論文發表競賽優勝 。 

-2012年指導林姓研究生榮獲"第十七屆生化工程研討會"壁報論文發表競賽優勝。  

-2012年指導譚姓研究生同學榮獲"第五屆桃園四校生命科技領域聯合學術研討會"論文海報競賽-生技工程組特優(第一名)。 

-2012年指導吳姓研究生同學榮獲"第五屆桃園四校生命科技領域聯合學術研討會"論文海報競賽-生技工程組優選(第二名)。 

-2012年榮獲執行『元智大學與亞東紀念醫院學術合作先期研發專題計畫』。 

-2011年獲邀參與Gorden Research Conference- Plant metabolic Engineering (Poster Presenter)。 

-2010年指導研究生顧同學榮獲 The 16th Symposium of Young Asian Biochemical Engineers' Community 最佳壁報獎(Genetic, protein and metabolic engineering )。 

-2009年榮獲中央研究院獎勵國內學人來院短期訪問研究。 

-2007年榮獲"第二十五屆中華植物學會暨植物科學與生質能源前瞻學術研討會"優良壁報論文獎。 

-Travel Grant Award from graduate student council of University of Florida, 2006, Gainesville, Florida, U.S.A. (May 2006) 

-Graduate School Travel Grant Award in Plant Biology 2005, American Society of Plant Biologists, Seattle, Washington, U.S.A. (July 2005) (全美植物生理學最大會議)

-Best-Presentation Award at state-wide workshop for Plant Molecular & Cellular Biology, Daytona, Florida, U.S.A. (May 2005)